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Then a biotinylated detection antibody specific for IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Mouse UCP2 in the samples is then determined by comparing the OD of the samples to the standard curve
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-Beta
The concentration of Sheep TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Human CLL1 in the samples is then determined by comparing the OD of the samples to the standard curve
Rabbit ET-2 (Endothelin 2) ELISA Kit SPENCDI Then a biotinylated detection antibodyThe Rabbit (ET 2) Endothelin 2 ELISA Kit measures Endothelin 2 in Rabbit samples. The plate has been pre coated with Rabbit ET 2 antibody. ET 2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit ET 2 Antibody is added and binds to ET 2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ET 2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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