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Calculate the concentration of PDK4 in the sample by plotting standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FGL1
And then biotinylated Human OLFM1 Antibody is added and binds to OLFM1 in the sample
And then biotinylated Human PGAM2 Antibody is added and binds to PGAM2 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated Nos1 antibody
Rat OPN (Osteopontin) ELISA Kit Packaging:96 wells Calculate the concentration of PDK4The Rat OPN (Osteopontin) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPN and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and
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