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Calculate the concentration of PACAP in the sample by plotting standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb3
When the sample containing Carbonic Anhydrase XIV is added to the plate
it binds with IL-3 bound to precoated antibody
The plate has been pre-coated with Rat RELN antibody
Mouse TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit HEL168 Calculate the concentration of PACAPThe Mouse TNF Alpha (Tumor Necrosis Factor Alpha) ELISA Kit measures TNF Alpha in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
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