Rat PDGFA (Platelet Derived Growth Factor Subunit A) ELISA Kit Packaging:96 wells IL25 present in the sample

IL25 present in the sample is added and binds to antibodies coated on the wells

Then Streptavidin-HRP is added and binds to the Biotinylated AGRP antibody

Universal MDA in the sample or standard competes with a fixed amount of Universal MDA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal MDA

Substrate solution is then added and color develops in proportion to the amount of Human ELA1

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig SCF

Rat PDGFA (Platelet Derived Growth Factor Subunit A) ELISA Kit Packaging:96 wells IL25 present in the sampleThe Rat PDGFA (Platelet Derived Growth Factor Subunit A) ELISA Kit measures PDGFA in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PDGFA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add