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PTX3 present in the sample is added and binds to antibodies coated on the wells
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse CLU are added to the micro ELISA plate wells
then it binds with nuclear-Ab bound to precoated antigen
Then Streptavidin-HRP is added and binds to the Biotinylated COLQ antibody
Calculate the concentration of TNFRSF4/OX40 in the sample by plotting standard curve
Rabbit TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit Packaging:96 wells PTX3 present in the sampleThe Rabbit TIMP 2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit measures TIMP 2 in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TIMP 2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.
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