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Substrate solution is then added and color develops in proportion to the amount of Human STRUM
The plate has been pre-coated with Rat UBIAD1 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL17F
And then biotinylated Human STUB1 Antibody is added and binds to STUB1 in the sample
it binds with AKR1C4 bound to precoated antibody
Human MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human MCP 1 (Monocyte Chemotactic Protein 1) ELISA Kit measures MCP 1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MCP 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
