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The concentration of GSK3α in the sample was calculated by drawing a standard curve
RAB27B present in the sample is added and binds to antibodies coated on the wells
a horseradish peroxidase conjugated mouse anti-Mouse CD34 Antibody is added to the wells and binds to CD34 in the sample
TJP1 present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Mouse MIF
Mouse IL-27 (Interleukin 27) ELISA Kit Packaging:96 wells The concentration of GSK3α inThe Mouse IL 27 (Interleukin 27) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse IL 27. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL 27 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
