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Then a biotinylated detection antibody specific for Human HP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Keratin 16 is expressed in keratinocytes
Then Streptavidin-HRP is added and binds to the Biotinylated CPE antibody
Then Streptavidin-HRP is added and binds to the Biotinylated EGLN1 antibody
HIF-1Alpha Mouse Monoclonal Antibody (Mix-mA)
Mouse FSH (Follicle-stimulating Hormone) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Mouse (FSH) Follicle stimulating Hormone ELISA Kit measures follicle stimulating hormone in Mouse samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB
