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Calculate the concentration of CD28 in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated ASRGL1 antibody
Then a biotinylated detection antibody specific for Human CD5L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated CASK antibody
The microplate provided in this kit has been precoated with anti RTEL1 antibody
Guinea pig HHIP (Hedgehog Interacting Protein) ELISA Kit Packaging:48 wells Calculate the concentration of CD28The Guinea pig HHIP (Hedgehog Interacting Protein) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig HHIP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig HHIP and Avidin Horseradish Peroxidase (HRP) conjugate are added
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