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Substrate solution is then added and color develops in proportion to the amount of Human ZC3H4
Universal DA in the sample or standard competes with a fixed amount of Universal DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DA
The assay plate has been pre-coated with mouse anti-Human IL-3R alpha monoclonal antibody
Only those wells that contain Porcine IL-2
GLI1 present in the sample is added and binds to antibodies coated on the wells
Rabbit TRX (Thioredoxin) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rabbit TRX (Thioredoxin) ELISA Kit measures TXN in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TXN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
