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the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of progesterone present
NUDT1 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated PLXNA4 antibody
only those wells that contain Human PMP22
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT2
Goat MT (Melatonin) ELISA Kit SFTP1 the TMB substrate (enzyme substrate)The Goat MT (Melatonin) ELISA Kit measures MT in Goat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with MT. MT in the sample or standard competes with a fixed amount of biotin labeled MT for sites on a pre coated antibody specific to MT. Free components are washed off. After adding HRP Streptavidin
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