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The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL1a
Then Streptavidin-HRP is added and binds to the Biotinylated COX6C antibody
and the color intensity is directly proportional to the amount of Human VNN2 present
The concentration of Human SP-D in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Rat OT Antibody is added and binds to OT in the sample
Human LAMA3 (Laminin Subunit Alpha-3) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Human (LAMA3) Laminin Subunit Alpha 3 ELISA Kit measures Laminin Subunit Alpha 3 in samples. The plate has been pre coated with Human LAMA3 antibody. LAMA3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LAMA3 Antibody is added and binds to LAMA3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LAMA3 antibody. After incubation unbound Streptavidin HRP is washed away
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