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a capture antibody-PSMA4-biotin-detection antibody complex was formed
a capture antibody-RELB-biotin-detection antibody complex was formed
TFF3 present in the sample is added and binds to antibodies coated on the wells
The concentration of Rat PTX3 in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human PGAM1
Mouse TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit ERBB a capture antibody-PSMA4-biotin-detection antibody complexThe Mouse TFF3 (Trefoil Factor 3, Intestinal) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse TFF3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TFF3 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each
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