Mouse RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini-Sample) Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Mouse TJP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

a horseradish peroxidase conjugated mouse anti-Human SAA4 Antibody is added to the wells and binds to SAA4 in the sample

The concentration of ANGPTL-4 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values

Only those wells that contain Human sTfR1

Human Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol

Mouse RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini-Sample) Packaging:96 wells Then a biotinylated detection antibodyThis Mouse RANL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini Sample) uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to RANL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RANL and Avidin Horseradish Peroxidase (HRP) conjugate are added