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Crh in the sample or standard competes with a fixed amount of biotin-labeled Crh for sites on a pre-coated antibody specific to Crh
And then biotinylated Mouse SHC1 Antibody is added and binds to SHC1 in the sample
MYBL2 present in the sample is added and binds to antibodies coated on the wells
Calculate the concentration of FST in the sample by plotting standard curve
Substrate solution is then added and color develops in proportion to the amount of Canine ANG-2
Rat CASR (Calcium Sensing Receptor) ELISA Kit Packaging:96 wells Crh in the sample orThe Rat CASR (Calcium Sensing Receptor) ELISA Kit measures CASR in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CASR antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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